Share your computing skills tips and tricks with other members. Contribute and help others.





Google
 

Sunday, June 29, 2008

Isolation of early Hematopoietic Stem cells from Murine Yolk Sac and AGM

Isolation of early Hematopoietic Stem cells from Murine Yolk Sac and AGM
Video Experiment

Kelly Morgan, Michael Kharas, Elaine Dzierzak, D. Gary Gilliland

In the mouse embryo, early hematopoiesis occurs simultaneously in multiple organs, which includes the yolk sac and aorta-gonad-mesonephros region. These regions are crucial in establishing the blood system in the embryos and leads to the eventual movement of stem cells into the fetal liver and then development of adult stem cells in the bonemarrow. Early hematopoietic stem cells can be isolated from these organs through microdissection of the embryo followed by flow cytometric sorting to obtain a more pure population. It remains unclear how these stem cell populations contribute to the fetal and adult stem cell pool. Also, our lab investigates how early stem cells functionally differ from fetal and adult hematopoietic stem cells. Furthermore, our lab sorts different populations of hematopoietic stem cells and test their functional role in the context of a variety of genetic models. In this video, we demonstrate the micro-dissection procedure we commonly use and also show the results of a typical FACS plotfter isolating these rare populations, it is possible to perform a variety of functional assays including: colony assays and bone marrow transplants.

view here
http://www.jove.com/index/details.stp?ID=789

Labels:

Pyrosequencing: A Simple Method for Accurate Genotyping

Pyrosequencing: A Simple Method for Accurate Genotyping
Video Experiment

Cristi King, Tiffany Scott-Horton
Department of Internal Medicine, Washington University of St. Louis

Pharmacogenetic research benefits first-hand from the abundance of information provided by the completion of the Human Genome Project. With such a tremendous amount of data available comes an explosion of genotyping methods. Pyrosequencing(R) is one of the most thorough yet simple methods to date used to analyze polymorphisms. It also has the ability to identify tri-allelic, indels, short-repeat polymorphisms, along with determining allele percentages for methylation or pooled sample assessment. In addition, there is a standardized control sequence that provides internal quality control. This method has led to rapid and efficient single-nucleotide polymorphism evaluation including many clinically relevant polymorphisms. The technique and methodology of Pyrosequencing is explained.

view here
http://www.jove.com/index/Details.stp?ID=630

Labels:

Sunday, June 22, 2008

Cardiothoracic Surgery Network Congenital videos

Cardiothoracic Surgery Network is the leading source of educational and scientific research information for cardiothoracic surgeons, allowing them to advance their skills and stay current with new innovations in the field.

- Congenital videos -

Valve-Sparing Aortic Root Replacement in a six year old Child with Bicuspid Aortic Valve and Loeys-Dietz Syndrome by Luca A. Vricella, MD, FACS and Duke Edward Cameron, MD
28-April-2008

Aortic Translocation for d-TGA, VSD, and Subpulmonary Stenosis by Frank A. Pigula, MD
06-February-2008

Ebstein's Anomaly Repair (Modified Danielson Technique) by Vijit Koshy Cherian, MD, Alpha Kavunkai, MD, Jayavelan Ramkumar, MD, and Gangahanumaiah Shivanand, MD
23-January-2008

ALCAPA Repair by Martin Kostolny, MD and Victor Tsang, MD
20-November-2007

Surgical Repair of Total Anomalous Pulmonary Venous Drainage by Mahesh S. Sharma, MD, Victor T. Tsang, MD, Muhammed Nuri, MD, and Martin Kostolny, MD
15-June-2007

Modified Senning and Rastelli Procedures with Bidirectional Cavopulmonary Anastomosis by Kristine J. Guleserian, MD, Reenu S. Eapen, MD, Michael Spann, MD, et al STSA 2006
25-May-2007

Bi-Directional Glenn Shunt by A. Sampath Kumar, MD and Sachin Talwar, MS, MCh
24-April-2007

Repair of the Tetralogy of Fallot with Absent Pulmonary Valve by Karl F. Welke, MD, Irving Shen, MD and Ross M. Ungerleider, MD STSA 2006
16-January-2007

Total Aortic Arch Replacement for Recurrent Mycotic Pseudo-Aneurysm in 5 Year Old Following Fontan Procedure by Christian L. Gilbert, MD STSA 2006
14-December-2006

Switch - Procedure by Pascal R. Vouhe, MD and Alexander Kadner, MD
03-August-2006

Hypoplastic Left Heart Syndrome with Atresia of the Ascending Aorta by Joseph Forbess, MD, Reenu S. Eapen, MD and William A. Scott, MD STSA 2005
14-April-2006

Norwood Procedure by Pascal R. Vouhe, MD and Alexander Kadner, MD
22-February-2006

Transatrial & Transpulmonary Closure of VSD by A. Sampath Kumar, MD
25-October-2005

Repair of Total Anomalous Pulmonary Venous Connection by A. Sampath Kumar
13-June-2005

Repair of Ebstein's Anomaly by A. Sampath Kumar
13-June-2005

Modified Konno Procedure by Irving Shen, MD and Ross Ungerleider, MD
01-February-2005

ASD Closure Through Right Thoracotomy by A. Sampath Kumar, MD
10-June-2004

Thorascopic Cardiac Procedures in Children by Redmond Burke, MD
25-February-2000

Repair of Complete Atrioventricular Defect Using Two-Patch Technique by Jaroslav Stark, MD
31-March-1999

Labels:

Cardiothoracic Surgery Network Cardiac videos

Cardiothoracic Surgery Network is the leading source of educational and scientific research information for cardiothoracic surgeons, allowing them to advance their skills and stay current with new innovations in the field.

- Cardiac videos-

Repair of Mitral Valve Prolapse Using Artificial Chordal Replacement by J. Scott Rankin, MD, Ricardo E. Orozco MD and Calvin A. Burrichter, MD American Heart Association 2007
28-May-2008

Robotic Mitral Valve Repair Using the Haircut Technique by Michael W. A. Chu, MD, FRCSC, Karen Gersch, M.D., L. Wiley Nifong, and W. Randolph Chitwood, Jr STSA 2007
19-March-2008

Combined Open Proximal and Stent Graft Distal Repair for Complex Arch Aneurysm by Andreas Zierer, MD, Luis A. Sanchez, MD and Marc R. Moon, MD STSA 2007
19-March-2008

Aortic Valve Replacement with Stentless Autologous Gluteraldehyde Treated Pericardium by A. Sampath Kumar, MD and Carlos MG Duran, MD, PhD
16-March-2008

Mitral Valve Repair in Barlow's Disease by V. Bautista-Hernandez, MD and V. Roques-Escolar
18-December-2007

Minimally Invasive Beating Heart MVR in Severe Dilated Cardiomyopathy by Saquib Masroor, MD, MHS
19-November-2007

Thoracoscopic Mitral Valve Repair in Dilated Cardiomyopathy: A Case of Chordal Tethering by Saqib Masroor, MD, MIIS and John Alexander, MD
21-August-2007

Subclavian Artery to Left Anterior Descending Artery By-Pass via Left Anterior Small Thoracotomy ("Last Procedure") by Domenico Calcaterra, MD, Marco Ricci, MD, Anthony L. Panos, MD, et al
06-August-2007

Robotically Assisted Repair of a Partially Avulsed Papillary Muscle Secondary to Blunt Trauma by Clifton C. Reade, MD, Alan P. Kypson, MD, Friedrich W. Mohr, MD, and W. Randolph Chitwood Jr., MD STSA 2006
19-January-2007

Surgical Ventricular Restoration by Lois Nwakanma, MD, Jason Williams, MD, Nishant Patel, MD, and John V. Conte, MD STSA 2006
17-January-2007

Repair of an Extent II Thoracoabdominal Aortic Aneurysm in a Marfan's Patient with Previous Thoracic Aortic Stenting by Hazim J. Safi, MD, Anthony L. Estrera, MD, Eyal Porat, MD, et al STSA 2006
20-December-2006

DaVinci(TM) Robotic Mitral Valve Repair for Anterior Leaflet Bacterial Endocarditis by Evelio Rodriguez, MD, Simon C. Moten, MD, L. Wiley Nifong, MD, and W. Randolph Chitwood Jr., MD STSA 2006
18-December-2006

Valve-Sparing Aortic Root Replacement for Marfan Syndrome with Type A Dissection by Andreas Zierer, MD and Marc R. Moon, MD STSA 2006
15-November-2006

Surgical Treatment of Multiple Intra-Cardiac and Pulmonary Artery Tumor Implants Embolic from Uterine Intravascular Leiomyomatosis by Raul Garcia Rinaldi, MD, Jose Perez Hernandez, MD, Alma R. Corbala, MD, et al
27-September-2006

Repair of Rheumatic Mitral and Aortic Valve (AV + MV Repair) by A. Sampath Kumar, MD
15-June-2006

Thoracoscopic LITA Harvesting by Tomasz Hrapkowicz, MD and Gianluigi Bisleri, MD
22-May-2006

Repair of Rheumatic Tricuspid Valve Disease by A. Sampath Kumar, MD
16-March-2006

Myocardial Revascularisation (CABG) by A. Sampath Kumar, MD
18-January-2006

Mitral Valve Replacement & Tricuspid Valve Repair (Transeptal Approach) by A. Sampath Kumar, MD
18-January-2006

Thoracic Aortic Aneurysm Repair Via Bilateral Thoracosternotomy (Clamshell) Approach by Jayesh Dhareshwar, MD and Thoralf M. Sundt III, MD STSA 2005
22-November-2005

Off-Pump Hybrid Total Arch Exclusion: The Antegrade Deployment Approach by George Tolis Jr., MD and Richard M. Green, MD
11-November-2005

Closed Mitral Valvotomy (CMV) by A. Sampath Kumar, MD
25-October-2005

Direct Axillary Cannulation for Cardiopulmonary Bypass by George Tolis Jr., MD
06-September-2005

Homograft Valve Banking (With Cryopreservation) by A. Sampath Kumar, MD
12-August-2005

Bentall Operation with Aortic Homograft by A. Sampath Kumar, MD
18-July-2005

Pericardiectomy for Chronic Constrictive Pericarditis by A. Sampath Kumar, MD
13-July-2005

Right Atrial Myxoma by Edward Bender, MD
15-June-2005

Total Arch Replacement with Stage I Elephant Trunk Procedure by George Tolis Jr., MD
18-May-2005

Videoscopic Second Repair of Mitral Valve (Barlow's) Using Two Annuloplasty Bands by Saquib Masroor, MD, L. Wiley Nifong, MD and W. Randolph Chitwood Jr., MD, FACS
25-April-2005

Retro-Cardiac Ascending Aorta to Descending Aorta Bypass by Clifton C. Reade, MD, Theodore C. Koutlas, MD, FACS, Curtis E. Bower, MD, et al
19-April-2005

Replacement of the Descending Thoracic Aorta in an Animal: A Totally Robotic Procedure by Eyal E. Porat, MD, Peter D. Herrera, BS, Roy Sheinbaum, MD, et al
28-March-2005

Transventricular Fixation of Papillary Muscles for Mitral Homograft Implantation in Tricuspid Position by Matthias Kirsch, MD, N. Costin Radu, MD and Daniel Loisance, MD
07-March-2005

Aortic Valve Replacement in a Beating Heart by Pierluca Lombardi, MD, Marco Ricci, MD, Fuad Moussa, MD, and Tomas Salerno, MD
22-November-2004

Mitral Valve Replacement with a Prosthetic Valve by A. Sampath Kumar, MD
27-September-2004

Repair of Myxomatous Mitral Valve by A. Sampath Kumar, MD
16-August-2004

Trans Aortic Double Valve Replacement by A. Sampath Kumar, MD
16-August-2004

Valve Sparing Reimplantaion Procedure with the Gelweave Valsalva Graft by Sidney Chocron, MD
21-July-2004

Valve Sparing Aortic Root Replacement with Aortic Valve Re-implantation (David I Procedure) by George Tolis Jr., MD
21-July-2004

MVR on a Beating Heart Through Right Thoracotomy by Ciro Campanella, MD and Ali Asgar A. Behranwala, MD
24-June-2004

Malignant Tachyarrhythmia Cured by Tumor Resection in a Patient with Giant Ventricular Lipoma by Erik Beyer, MD
23-June-2004

Sub-Total Arch Replacement by George Tolis Jr., MD
18-June-2004

Valve Sparing Aortic Root Replacement for Marfan Syndrome by David G. Affleck, MD and Marc R. Moon, MD
14-June-2004

Pulmonary Autograft AVR by A. Sampath Kumar, MD
27-May-2004

Off-Pump All-Arterial Coronary Bypass: Surgical Revascularization for the New Millennium by Jacob DeLaRosa, MD, Erez Sharoni, MD and John D. Puskas, MD
26-May-2004

Closed-Chest Thoracoscopic Surgical Treatment of Atrial Fibrillation by Claudio Muneretto, MD and Gianluigi Bisleri, MD
24-May-2004

Robotic MVR Utilizing Double Arm Nitinol U-Clips by Clifton C. Reade, MD, Curtis E. Bower, MD, B. Marcus Bailey, MD, et al
14-April-2004

Combining Robotic MVR and Microwave Atrial Fibrillation Ablation by Clifton C. Reade, MD, Curtis E. Bauer, MD, Alan P. Kypson, MD, et al
15-March-2004

Thorascopic Left Ventricular Lead Placement for Resynchronization Therapy by James R. Edgerton, MD, Mitchell Magee, MD, Michael Mack, MD, and Todd Dewey, MD
04-February-2004

Aortic Valve Replacement with a Homograft (Scallopped Subcoronary Techique) by A. Sampath Kumar, MD
16-December-2003

Mitral Valve Replacement with a Pulmonary Autograft (Ross II Procedure) by A. Sampath Kumar, MD
16-December-2003

Mitral Valve Replacement with a Bioprosthesis by A. Sampath Kumar, MD
16-December-2003

Mitral Valve Repair by A. Sampath Kumar, MD
16-December-2003

Minimally Invasive-Robotic Microwave Atrial Fibrillation Ablation with Mitral Valve Repair by Clifton C. Reade, MD, Curtis E. Bauer, MD, Alan P. Kypson, MD, et al
02-July-2003

Aortic and Mitral Valve Replacement with Annular Reconstruction for Native Valve Endocarditis by Nilto DeOliveira, MD and Marc R. Moon, MD
02-July-2003

Aortic Valve Replacement with a Bioprosthesis by A. Sampath Kumar, MD
16-February-2003

Off-pump Microwave Ablation of Chronic Atrial Fibrillation by David C. Kress, MD
19-August-2002

Mitral Valve Repair and Radio Frequency Ablation of the Left Atrium by Donald A. Sisto, MD
24-July-2002

Mitral Valve Anatomy and Morphology by G. L. van Rijk-Zwikker, MD
18-July-2002

Aortic Valve Replacement with Freestyle Bioprosthesis by Donald B. Doty, MD
20-April-2001

Early Experience with a Quadrileaflet Stentless Mitral Valve by Britt Hofmann, MD
17-April-2001

Left ventricular outflow tract obstruction due to mitral valve anomaly by R. Calabrò, MD
28-March-2001

Isolated four-chamber working swine heart model by Edward Chinchoy, MD
28-March-2001

InVivo Flow Dynamics by Shiva Sharma, MD
26-March-2001

Autotransplantation for Cardiac Sarcoma by Michael Reardon, MD
14-November-2000

Valve Repair in Mitral Regurgitation Complicated by Severe Annulus Calcification by Choi-Keung Ng, MD
02-November-2000

CABG in the Conscious Patient Without Endotracheal General Anesthesia by Haldun Y. Karagoz, MD
25-April-2000

Carinal Resection With Cardiopulmonary Bypass by Rodney J. Landreneau, MD
17-April-2000

The Implantation Technique of the Quadrileaflet Stentless Mitral Valve by Romuald Cichon, MD
14-April-2000

Mitral Homograft by Neal Kon, MD
30-March-2000

Are We Implanting Half Valves? The Effect of Loss of Sinus Wall Compliance Upon Aortic Valve Longevity by Francis Robicsek, MD
29-March-2000

Thoracoabdominal Aortic Aneurysm Repair by Joseph Coselli, MD
01-March-2000

Technical Aspects of Double Skeletonized IMA Grafting by Jacob Gurevitch, MD, Amir Kramer, MD, Chaim Locker, MD, et al
14-December-1999

Minimally Invasive Valve Surgery: Operative Technique for Mitral and Aortic Operative Technique for Aortic Valve Replacementalve Replacement by Lawrence H. Cohn, MD
19-April-1999

Radial Approach for Atrial Fibrillation by Takashi Nitta, MD, Yosuke Ishii, MD, Hidetsugu Ogasawara, MD, et al
16-April-1999

Coronary Artery Endarterectomy for Extensive In-Stent Restenosis by Albert J. Pfister, MD
26-March-1999

Assessment of Mitral Regurgitant Jets by Three-Dimensional Color Doppler by Raffaele De Simone, MD, Gerald Glombitza, PhD, Christian-Friedrich Vahl, MD, et al
23-March-1999

Ventricular Restoration for Akinesia (Modified Dor Procedure): Myocardial Protection and Surgical Approach by C. L. Athanasuleas, MD and G. D. Buckberg, MD
12-March-1999

Complete Coronary Artery Revascularization on Beating Heart by KiHong Kwon, Robert S. Firpo and Thomas Tzeng
12-March-1999

Mitral Valve Replacement with Mitral Homograft by Donald B. Doty, MD, Roger C. Millar, MD and Christophe Acar, MD
11-November-1998

Aortic Root Enlargement and Replacement with Aortic Homograft by Donald B. Doty, MD
11-November-1998

The Ross Procedure: Supported Root by Donald B. Doty, MD
09-November-1998


Labels:

Cardiothoracic Surgery Network Thoracic videos

Cardiothoracic Surgery Network is the leading source of educational and scientific research information for cardiothoracic surgeons, allowing them to advance their skills and stay current with new innovations in the field.

You can view videos in flash format

- Thoracic videos-

Repair of a Symptomatic Bochdalek Hernia in the Adult by John Alfred Carr, MD
31-March-2008

Left Thoracoscopic Thymectomy with Da Vinci Robotic System in Patient with Non-thymomatous Myasthenia Gravis by Federico Rea, MD, Giuseppe Marulli, MD and Luigi Bortolotti, MD
19-March-2008

Thorascopic Resection of a Posterior Mediastinal Neurogenic Tumor by John Alfred Carr, MD
27-February-2008

Complete Lymph Node Dissection Under VATS for Lung Cancer by Hyun-Sung Lee, MD, PhD, Suk-Won Song, MD, Jon Mog Lee, MD, et al STSA 2006
17-November-2006

Open Extraction of Incorporated Tracheal Stent by Albert S. Y. Chang, MD, Thomas R. Gildea, MD and Sudish C. Murthy, MD, PhD
24-March-2006

Minimally Invasive Management of Mediastinal Parathyroid Adenomas by James Cohen, MD, Chris Komanapalli, MD and Mithran Sukumar, MD
06-March-2006

Bilateral Sequential Lung Transplantation for Pulmonary Fibrosis by Cliff K.C. Choong, MD, Bryan Fitch Meyers, MD and G. Alexander Patterson, MD Presented 2005 STSA
12-December-2005

Bilateral Sequential Lung Transplantation for Cystic Fibrosis by Cliff K. Choong, MD, Bryan F. Meyers, MD and G. Alexander Patterson, MD
08-August-2005

Donor Lung Procurement by Cliff K. Choong, MD, Bryan F. Meyers, MD and G. Alexander Patterson, MD
08-August-2005

Left Carinal Pneumonectomy with Partial Resection of Left Atrium on Cardiopulmonary Bypass by Hon Chi Suen and Bill B. Daily
01-February-2005

Tracheobronchial Translocation by John H. Calhoon, MD, R. Brent New, MD and Luis Angel, MD
17-May-2004

Cine MRI of a Giant Carcinoid Tumor by S. Fischer, MD
15-November-2000

Carinal Resection With Cardiopulmonary Bypass by Rodney J. Landreneau, MD
17-April-2000

The Dartevelle Approach to Tumors by Joshua Sonett, MD
03-March-2000

Tracheal Homograft Reconstruction by Jeffrey Jacobs, MD
02-February-2000

Superior Video-Assisted Lobectomy Dissection Techniques by Todd L. Demmy, MD
19-May-1999




Labels:

 



Privacy Policy